Time-resolved fluorometry of red blood cell membrane conformational changes in a population exposed to low-dose radiation

Abstract

Using subnanosecond laser spectrofluorometry the spectral and polarization time-resolved characteristics of 1-phenylnaphthylamine (1-AN) fluorescent probe in phospholipid bilayer and red blood cell (RBC) membranes have been studied. It is shown that the electronic spectra of probe in model membranes inhomogeneously broadened. Inhomogeneous broadening affect significantly the spectroscopic properties of probe in membranes, such as the time-dependent fluorescence Stokes shift, the dependence of the fluorescence instantaneous spectra and fluorescence kinetics on excitation wavelength. The obtained results prove the existence of the earlier unknown effect of the wavelength- dependent rotation of a probe in phospholipid bilayer and RBC membranes.