Abstract
Cryogenic procedures are fundamental tools in modern biology, e.g. for conservation or purification of biological materials. The processes occurring in biological cells and tissues during freezing and thawing are subject to ongoing research. Optimization of cell survival rates demands the development and evaluation of exactly defined temperature profiles. 4D-DMD-microscopy is capable of imaging these highly dynamic processes with high spatial and temporal resolution, utilizing well established staining procedures for differentiating structures of interest.
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