Abstract
Dynamic arrays of microbeads and cells offer great flexibility and potential as platforms for sensing and manipulation applications in various scientific fields, especially biology and medicine. Here, we present a simple method for assembling and manipulating dense dynamic arrays based on time-shared scanning optical tweezers with a microlens array. Three typical examples, including the dynamic and simultaneous bonding of microbeads in real-time, are demonstrated. The optical design and the hardware setup for our approach are also described.
Highlights
Microsystems and nanosystems for biomedical fields, such as bio-MEMS and Lab-on-a-Chip, are areas of intensive research [1, 2]
Microarrays represented by DNA chips, which are well established for bio-sensing applications such as DNA detection and SNP genotyping, are valuable tools for fundamental studies in biology and medicine
Compared with static microarrays such as a DNA chip, dynamic microarrays using lasertrapped mobile substrates, usually consisting of microbeads coated with biomolecules, biochemicals, or cells, offer greater flexibility and potential as platforms for sensing/manipulation applications in various scientific fields as well as biomedical fields [3,4,5,6,7]
Summary
Microsystems and nanosystems for biomedical fields, such as bio-MEMS and Lab-on-a-Chip, are areas of intensive research [1, 2]. Unlike the time-shared scanning (TSS) approach [6], the HOT/GPC-based approaches are suitable for manipulating a large number of microbeads to form dense dynamic microbead arrays, because no dwell time is required for stable multiple optical traps [12]. These approaches require a spatial light modulator (SLM), which is an expensive device and needs complicated control procedures for optical engineers and expert programmers. The optical design and inexpensive hardware setup for our demonstrations are described
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
