Abstract
RNA's unique folds and motions enable many of its newly discovered functions, yet RNA self-assembly and conformational dynamics are not well understood. Here, we use microfluidic mixing to trigger the Mg induced folding of tP5abc; a representative of the common RNA motif the three helix junction. Time-dependent conformations are measured using time-resolved small angle x-ray scattering (SAXS), and analyzed by pairing ensemble decomposition with all-atom simulations. Detailed structural ensembles, derived at crucial stages of folding, reveal distinct phases of tP5abcs journey to the native state. Many of these phases rely on the presence of Mg ions, and underscore their unique and varying role(s) in guiding RNA through a complex and rugged landscape.
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