Time-resolved multispectral imaging based on an adaptive single-pixel camera.

Florian Rousset,Andrea Farina,Françoise Peyrin,Cosimo D’Andrea,Gianluca Valentini,Nicolas Ducros

doi:10.1364/oe.26.010550

Abstract

Time-resolved multispectral imaging has many applications in different fields, which range from characterization of biological tissues to environmental monitoring. In particular, optical techniques, such as lidar and fluorescence lifetime imaging, require imaging at the subnanosecond scales over an extended area. In this paper, we demonstrate experimentally a time-resolved multispectral acquisition scheme based on single-pixel imaging. Single-pixel imaging is an emerging paradigm that provides low-cost high-quality images. Here, we use an adaptive strategy that allows acquisition and image reconstruction times to be reduced drastically or full basis scans. Adaptive time-resolved multispectral imaging scheme can have significant applications in biological imaging, at scales from macroscopic to microscopic.

Highlights

Imaging phenomena that occur atnanosecond time scales have a variety of applications that range from lidar to fluorescence lifetime imaging (FLIM)
For the recovery of FLIM fluorescence lifetime maps, fluorescence decay needs to be measured on the picosecond to nanosecond time scale
We experimentally demonstrate time-resolved multispectral imaging by means of a single-pixel camera (SPC) using an adaptive scheme

Summary

Introduction

Imaging phenomena that occur at (sub)nanosecond time scales have a variety of applications that range from lidar to fluorescence lifetime imaging (FLIM). For the recovery of FLIM fluorescence lifetime maps, fluorescence decay needs to be measured on the picosecond to nanosecond time scale. FLIM is one of the most important techniques in biological and materials studies [2, 3], as it can provide fundamental insight into local variations in pH, temperature, and ion concentrations. Beyond this temporal resolution, acquisition of the emission spectrum is fundamental to study photophysical properties of samples that can range from materials to devices [4]. There is a strong need for multi-dimensional (i.e., temporal, spectral, spatial) FLIM set-ups with picosecond resolution

Methods

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Conclusion