Time-resolved fluorescence depolarization techniques in model membrane systems. Effect of sterols and unsaturations.

Abstract

Application of time-resolved fluorescence depolarization techniques to the study of biological membrane structure and dynamics requires selective signal monitoring of specifically located intrinsic or extrinsic fluorophores. In such systems, at least to date, the fluorescence characteristics of the naturally occurring chromophores (e.g., aromatic amino acid residues, enzymatic reactional cofactors, polyenic fatty acids and sterols, chlorophyll, and retinal) are of limited use because of low and/or reabsorbed emitted intensities, low signal-to-noise ratio (e.g., light scattering), and so on.KeywordsAcyl ChainFluorescence AnisotropyFatty Acyl ChainFluorescence DepolarizationParinaric AcidThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.