Time-dependent gut microbiota analysis of juvenile Oreochromis niloticus by dietary supplementation of resveratrol.

Abstract

To evaluate the changes in bacterial diversity at various time points under resveratrol supplementation, we aimed to investigate the diversification of gut microbiota and the changes in total genetic diversity. We performed 16S rDNA gene sequencing at different time points (15, 30, and 45days) to analyze the gut microbiota of tilapia. Fusobacteria, Proteobacteria, and Bacteroidetes (15days) or Cyanobacteria (30 and 45days) were found to be the three most abundant phyla. Cyanobacteria (15 and 30days), Proteobacteria (15days), Firmicutes and Chlamydiae (30 and 45days), Planctomycetes (30days), Bacteroidetes, Actinobacteria, and Fusobacteria (45days) in the 0.05g/kg RES group increased as compared to that in the controls. Proteobacteria and Cyanobacteria significantly decreased and increased at 30 and 45days, respectively, while the reverse pattern was observed at 15days. The Bacteroidetes:Firmicutes and Proteobacteria:Cyanobacteria ratios were significantly increased (15 and 45days, P < 0.05) and decreased (30days, P < 0.05). RES supplementation did not affect the richness and diversity of the gut microbiota in tilapia. Our findings may contribute to the development of strategies for the management of diseases.