TIME DEPENDENT EVALUATION OF EFFICACY ON INHIBITION OF BACTERIAL AND FUNGAL GROWTH OF THREE DISINFECTANTS BY IMMERSION AND SPRAYING METHOD ON POLYVINYL SILOXANE IMPRESSION MATERIAL - AN IN-VITRO STUDY

Abstract

Aim:To determine the efficacy of three disinfectants-2% Glutaraldehyde,0.2% Peracetic acid,70%Isopropyl alcohol with time(3 minutes and 7 minutes) on Staphylococcus Sp. and Candida Sp. on polyvinyl siloxane impression material. Settings and Design:This was an in vitro comparative study. Materials and Methods:A standard inoculum of 0.5 McFarlandwas applied on the prepared polyvinyl siloxane disc surface.Discs were disinfected by immersion and spraying method for 3 minutes and 7 minutes.After that discs were collected in nutrient broth,vortexed and a small volume of the broth was centrifuged.It was thentransferred to test tube containing neutralizing agents of the corresponding disinfectants.0.1 ml from the test tube was transferred to culture plate and incubated to study thesurvivality of Staphylococcus Sp. and Candida Sp. Statistical Analysis Used:One way ANOVAtest was performed to check the mean reduction of microorganism in each group after disinfection by immersion and spraying.Inter group comparison of disinfection efficacy within certain time(3 minutes or 7 minutes) span was done by Tukeys post hoc test. Results:Percentage(%) of mean reduction of staphylococcus and candidas growth obtained from this studywere 99.99% by immersion and spraying method within 3 minutes and 7 minutes. Significance:Both immersion and spraying methods had similar reduction efficacy(99.99%) when compared with control.