Abstract
Cholinergic receptor-rich membranes from the electroplax of Torpedo californica have been partitioned between the upper phase and the interface of an aqueous two-phase system consisting of water, dextran and polyethylene glycol. The partitioning of membranes containing the cholinergic receptors was influenced by including polyethylene glycol-bound hexaethonium in the upper phase. The partition behaviour was studied by using the two-phase systems for counter-current distributions with a varying number of steps. The membranes showed time-dependent distributions, which is assumed to be due to changes in the internal distribution of membrane constituents and/or formation of larger membrane aggregates. Despite this effect good purification of the cholinergic membranes could be obtained. The consequences of the time-dependent effect for the partitioning of membranes within aqueous two-phase systems as well as the possibilities to reduce it are discussed.
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