Abstract
Platelet-rich plasma (PRP) contains bioactive cytokines to enhance tissue healing. The best PRP preparation protocol and timing of the treatment have not been determined yet. To screen the best-fit PRP, a 3-chamber co-culture device was developed. We hypothesized the concentrations of the cytokines from different PRPs in the co-culture plates had a high correlation with those in conventional 24-well culture plates at different time points. The concentrations of the cytokine from PRPs would be correlated with platelet concentrations. The correlation of transforming growth factor beta-1 (TGF-β1) and platelet-derived growth factor AB (PDGF-AB) in both devices were compared at 0, 24, 48, 72, and 96 h from two PRPs as well as that of platelet and cytokines concentrations. The results revealed that there was a moderate to high correlation in TGF-β1 concentrations between the 3-chamber co-culture and conventional culture device until 96 h. The correlation of PDGF-AB concentrations in both devices had moderate to high correlation in the first 24 h, and then it became modestly correlated from 48 to 96 h. A high correlation was found between platelet and TGF-β1 concentration at 96 h. However, they were modestly correlated in other time points. A negative or modest correlation was found between platelet and PDGF-AB concentration in all time points. In conclusion, TGF-β1 and PDGF-AB revealed a time-dependent manner of release at five time points. There is a moderate to high correlation of the TGF-β1 and PDGF-AB concentration in both devices at different time points. However, TGF-β1 and PDGF-AB concentrations are not always proportional to the platelet concentration of the PRPs.
Highlights
Platelet-rich plasma (PRP) is an autologous blood-derived product that is applied exogenously to various tissues
More than 40 commercial systems claim to concentrate whole blood into PRP, but a standardized preparation protocol has yet to be implemented [9]. It is unclear why PRP preparation is considered the optimal treatment for various cell types and that a “more is better” theory for the use of higher platelet concentrations could not be supported
We hypothesized that: (1) The concentration of cytokines from different PRPs in the 3-chamber co-culture device had a high correlation with those in the conventional culture wells at different time points; (2) The cytokine concentrations in different PRPs would be correlated with platelet concentrations
Summary
Platelet-rich plasma (PRP) is an autologous blood-derived product that is applied exogenously to various tissues. More than 40 commercial systems claim to concentrate whole blood into PRP, but a standardized preparation protocol has yet to be implemented [9] It is unclear why PRP preparation is considered the optimal treatment for various cell types and that a “more is better” theory for the use of higher platelet concentrations could not be supported. We hypothesized that: (1) The concentration of cytokines from different PRPs in the 3-chamber co-culture device had a high correlation with those in the conventional culture wells at different time points; (2) The cytokine concentrations in different PRPs would be correlated with platelet concentrations Such information could help clinicians to determine the in vitro best PRP preparation protocol by using the co-culture device without taking too much specimen during surgery. The different concentrations of cytokines from PRPs at different time points could provide help to determine the optimal timing of PRPs applications in clinical practice
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