Abstract
The time-correlated single photon counting (TCSPC) method [1] is one of the most widely applied tools for the determination of fluorescence lifetimes and is intensively used in basic research, biology, and medicine, due to its unique mix of properties. It combines speed, i.e., the ps and ns time scale, with ultrasensitivity (epitomised by single-molecule detection), and an unsurpassed dynamic range, enabling the researcher to study very intensely and very weakly emitting species simultaneously. A TCSPC system typically consists of a high-repetition rate laser, electronic modules, and a fast and sensitive detector, such as a photomultiplier tube (PMT), or more recently, a microchannel-plate-(MCP)-PMT. The MCP-PMT is distinguished by its very small transit time spread, resulting in instrument response functions (IRF) well below 20 ps
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