Time- and dose-dependent effects of Amyloid Beta 1-40 (Aß1-40) peptide on retinal ganglion cell survival: TUNEL assay

Abstract

Event Abstract Back to Event Time- and dose-dependent effects of Amyloid Beta 1-40 (Aß1-40) peptide on retinal ganglion cell survival: TUNEL assay Mohd Aizuddin Mohd Lazaldin1*, Igor N. Iezhitsa1, 2, 3, Renu Agarwal1, 2, Puneet Agarwal4, Methil K. Kutty2, Anna Krasilnikova1, 2, Nur Hidayati Mohd Sharif2 and Nafeeza Mohd Ismail1, 2 1 Universiti Teknologi MARA, Centre for Neuroscience Research/Faculty of Medicine, Malaysia 2 Universiti Teknologi MARA, Faculty of Medicine, Malaysia 3 Volgograd State Medical University, Research Institute of Pharmacology, Russia 4 International Medical University, School of Medicine, , Malaysia, Malaysia Amyloid beta (Aß) has been implicated in the pathogenesis of retinal damage in glaucoma and its deposition in retina is now widely known to be associated with retinal ganglion cell loss. However, it remains unclear whether the toxic effect of Aß1-40 on retina are affected in a dose- and time-dependent manner. The purpose of this study was to evaluate time- and dose-dependent effects of Aß1-40 peptide on retinal cell survival. Female Sprague Dawley rats (200-250 g) were divided into 5 groups. Groups 1 was intravitreally administered with vehicle (PBS), groups 2, 3, 4 and 5 were intravitreally administered with Aß1-40 in doses of 1.0 nmol, 2.5 nmol, 5 nmol and 10 nmol respectively. Apoptotic cells were detected by TUNEL labeling assay. Number of apoptotic cells was counted per 100 µm2 of ganglion cell layer. Seven days after exposure to Aß1-40, the number of apoptotic cells in groups 2, 3, 4 and 5 was 4.67, 6.32, 9.21 and 10.84 folds higher than the number of apoptotic cells in the control group (p<0.01). On day 14 postinjection, the number of apoptotic cells significantly increased, by 10.86, 12.50, 16.62 and 19.62 folds than it was in the control group (p<0.01). Subsequently, the number of apoptotic retinal showed a trend towards a decrease in their number and on day 30 of Aß exposure the number of apoptotic cells was only 7.13, 9.43, 13.85 and 15.62 folds higher in groups 2, 3, 4 and 5 compared to control group (p<0.01). In conclusion, intravitreally administered Aß1-40 reduces RGC survival in a dose-dependent manner. Additionally, it causes a time-dependent increase in retinal cell apoptosis up to day 14 followed by a trend towards recovery. Acknowledgements We would like to acknowledge the financial support from MOE (Malaysia) grant 600-RMI/FRGS 5/3 (111/2014) and UiTM grants 600-RMI/DANA 5/3/PSF (17/2015) and 600-IRMI/MyRA 5/3/LESTARI (0112/2016). Keywords: Apoptosis, in vivo, Amyloid beta, Toxicity, retinal ganglion cell Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Mohd Lazaldin M, Iezhitsa IN, Agarwal R, Agarwal P, Kutty MK, Krasilnikova A, Mohd Sharif N and Mohd Ismail N (2016). Time- and dose-dependent effects of Amyloid Beta 1-40 (Aß1-40) peptide on retinal ganglion cell survival: TUNEL assay. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00134 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Mr. Mohd Aizuddin Mohd Lazaldin, Universiti Teknologi MARA, Centre for Neuroscience Research/Faculty of Medicine, Sungai Buloh, Selangor, Malaysia, aizuddin1992@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Mohd Aizuddin Mohd Lazaldin Igor N Iezhitsa Renu Agarwal Puneet Agarwal Methil K Kutty Anna Krasilnikova Nur Hidayati Mohd Sharif Nafeeza Mohd Ismail Google Mohd Aizuddin Mohd Lazaldin Igor N Iezhitsa Renu Agarwal Puneet Agarwal Methil K Kutty Anna Krasilnikova Nur Hidayati Mohd Sharif Nafeeza Mohd Ismail Google Scholar Mohd Aizuddin Mohd Lazaldin Igor N Iezhitsa Renu Agarwal Puneet Agarwal Methil K Kutty Anna Krasilnikova Nur Hidayati Mohd Sharif Nafeeza Mohd Ismail PubMed Mohd Aizuddin Mohd Lazaldin Igor N Iezhitsa Renu Agarwal Puneet Agarwal Methil K Kutty Anna Krasilnikova Nur Hidayati Mohd Sharif Nafeeza Mohd Ismail Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.