Abstract

AbstractThanks to containing tumor‐related molecules, small extracellular vesicles (sEVs) are emerging as biomarkers in tumor liquid biopsy and commonly employed to diagnose a specific cancer. However, a pan‐cancer screening method for pre‐symptomatic patients is crucial to achieving the early cancer detection. Herein, a lipid‐protein capture system on herringbone (HB) microfluidic chip (HBEV‐Chips) to isolate multiple tumor‐derived sEVs is constructed. Phosphatidylserine (PS) is abnormally surface‐supposed on tumor‐derived sEVs and binds T‐cell immunoglobulin domain and mucin domain‐containing protein 4 (Tim4) in calcium‐dependent manner. PS+ sEVs isolated by the HBEV‐Chips is perform on liquid chromatography electrospray ionization tandem mass spectrometry and it is found that PS+ sEVs are highly correlated with tumor‐related pathway (P < 0.05), such as Cdc42 protein signal transduction, neuropilin signaling pathway, and Wnt signaling pathway. And it is further validated in clinical sample and found that PS+sEV number shows statistical difference between cancer patients and healthy donors in plasma (P < 0.01) and has efficient diagnostic power (area under curve = 0.86), suggesting PS+sEV as potential biomarker for pan‐cancer screening. The Tim‐4 functionalized device facilitates the early multiple cancer detection, providing the potential to be used as a rapid‐screening tool in clinical setting.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.