Abstract
Intermediate voltage electron microscopes are used in biological research to produce high quality images of relatively thick specimens, including whole cells prepared by critical point drying and sections up to several microns thick of embedded cells and tissues. Pairs of images with the specimen tilted by angles appropriate for the magnification and the specimen thickness can be viewed stereoscopically as a way to extract 3D information from these thick specimens. The tilts appropriate for stereo imaging of such thick specimens usually don't exceed +/-20 degrees. Quantitative 3D measurement and 3D reconstruction using tomography or computer graphic tracing methods give the highest accuracy results when the maximum range of tilt angles is available.
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