抗菌胜肽Tilapia Hepcidin 1-5對抑制癌症細胞之研究

Abstract

TH1-5 from antimicrobial peptides of marine organisms, tilapia (Oreochromis mossambicus) hepcidin. The results indicated that TH1-5, a synthetic 22-mer antimicrobial peptide, specifically inhibited human fibrosarcoma cell (HT1080 cell line) and human cervical epithelioid carcinoma cells (HeLa cell line) proliferation and migration. The way in which TH1-5 inhibited HT1080 and HeLa cell growth was then studied.TH1-5 inhibited HT1080 cell and HeLa cell growth in a concentration-dependent manner according to an MTT analysis, which was confirmed by a soft-agar assay and AO/EtBr staining. Scanning electron microscopy and Transmission Electron Microscope revealed that TH1-5 caused lethal membrane disruption in HT1080 cancer cells and HeLa cells, and a wound healing assay supported that TH1-5 decreased the migration of HT1080 cells and HeLa cells. A flow cytometric analysis, showed that the TH1-5 induced apoptosis. In addition, Bcl-xL，JNK, IL-6,IL-8, IL-10, IL-12, IL-13, IL-15, CAPN5, CTSG and INF-γ mRNA expression was upregulated after treatment with TH 1-5 in HeLa cell to compared the untreated group. And in HT1080 cell, IL-2, IL-8, CTSG and INF-γ mRNA expression was upregulated. These findings suggest a mechanism of cytotoxic action of TH1-5 and indicate that TH1-5 may be a promising chemotherapeutic agent against human fibrosarcoma cells and human cervical epithelioid carcinoma cells.