Thyroxine-induced changes in the glycosylation pattern and in brain and serum levels of rat α-fetoprotein

Abstract

1. 1. We have studied the effect of thyroid disfunction during the postnatal period, on the serum and brain levels of rat α-fetoprotein (AFP) and albumin. 2. 2. Hypothyroidism was induced by treatment of pregnant rats and their newborn pups with 2-mercapto-1-methylimidazole(methimazole). Hyperthyroidism was provoked in newborns by daily injections ofthyroxine (0.25 μg/g body wt) from the 3rd postnatal day to weaning. Impaired growth, lower brain size, altered behaviour and morphological features observed were according to an altered thyroid status. 3. 3. Hypothyroid rats showed a significantly reduction in serum AFP concentration (78% of control values at 8 days of age) and a slight increase in that of albumin. 4. 4. No significant changes were found in their brain AFP levels, while a somewhat higher brain albumin level could be appreciated. Thyroxine supplementation (0.2μg/rat/day) corrected most of these alterations. 5. 5. Hyperthyroidism induced a drastic fall in both serum and brain AFP levels (about 48% of the corresponding control values). 6. 6. Albumin concentration in serum was augmented significantly from the 12th postnatal day, but its brain levels did not change significantly. 7. 7. In hyperthyroid rats, a significant reduction (37% relative to controls) in the concanavalin A-non reactive microform of AFP, was observed. 8. 8. This alteration of the glycosylation pattern of AFP could be due to the inhibition by thyroxine of the activity of the hepatic enzyme GlcNAc-transferase III.