Abstract
Background and Aims: It has been suggested that thyrotropin-releasing hormone (TRH) may have functions beyond its fundamental regulating function. Previous studies have demonstrated that TRH promotes wound healing. We aimed to perform an in vitro study in fibroblasts to assess the role of TRH in wounds that frequently occur in diabetes. Thus, we investigated the effects of TRH in wound healing both under normoglycemic and hyperglycemic-conditions. Methods: L929 mouse fibroblast cell line was used in the experiments. The cell viability was confirmed with XTT. Then the scratch migration assay was used for assessing the wound healing. TRH was added to both control and hyperglycemia groups at 100 nM after the scratch was created. The wound areas were measured after 24 and 48 hours after the scratch. Results: TRH and/or hyperglycemia did not affect the cellular activity after 48 hours. TRH reduced the wound areas (42.6%) compared to the control (52.2%) after 24 hours. In the hyperglycemia group the wound area was 64.3% and 61.0% of initial area at the 24th and 48th hours respectively. TRH incubation reduced these wound areas to 55.2% and 47.1% of initial areas. Conclusion: TRH treatment accelerated wound healing in hyperglycemia, which indicates the positive effects of TRH in wounds, may occur in diabetes.
Highlights
Skin protects the body like a barrier against external factors such as chemical substances, UV rays and infections
In this study; we investigated the possible effects of thyrotropin-releasing hormone (TRH) on in vitro wound healing assays in both normoglycemic and hyperglycemic conditions
TRH incubation and/or hyperglycemia did not affect the cell after 48 hours (Figure 1)
Summary
Skin protects the body like a barrier against external factors such as chemical substances, UV rays and infections. Wound healing is a crucial process for the body. Diabetes is one of the most common causes of non-healing wounds. Even minor wounds are difficult to heal while susceptibility to infection increases. New, efficient and inexpensive treatment choices are needed for non-healing wounds in diabetes. We aimed to perform an in vitro study in fibroblasts to assess the role of TRH in wounds that frequently occur in diabetes. TRH reduced the wound areas (42.6%) compared to the control (52.2%) after 24 hours. In the hyperglycemia group the wound area was 64.3% and 61.0% of initial area at the 24th and 48th hours respectively. TRH incubation reduced these wound areas to 55.2% and 47.1% of initial areas. Conclusion: TRH treatment accelerated wound healing in hyperglycemia, which indicates the positive effects of TRH in wounds, may occur in diabetes
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