Abstract
A sensitive and specific radioimmunoassay for TRH is reported. Antibody was raised in a New Zealand white rabbit by immunization with TRH complexed to bovine thyroglobulin through bis-diazotized benzidine. The assay was capable of detecting below 7 pg of TRH in biological materials. Immunoreactive TRH was found in hypothalamic extracts from man, pig, rat, hamster, chicken, frog, snake, salmon, and in whole brain of the larval form of the primitive cyclostome lamprey, and in the head region of the protochordate amphioxus. The TRH levels in the frog hypothalamus were 1520–3620 pg/mg tissue, being 6–12 times the concentration detected in the rat hypothalamus. These findings indicate that TRH is distributed throughout the vertebrate kingdom, and has an ancient lineage. Immunoreactive (IR) TRH up to 764 pg/mg tissue was found in brain regions, other than hypothalamus, of the lower vertebrates, snake, frog, tadpole and salmon. Small but significant concentrations were also found in extrahypothalamic brain of th...
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