Abstract
Respiration of calf thyroid mitochondria, microsomes and homogenate was studied by conventional Warburg manometric techniques, and in a Clark oxygen electrode. Basal mitochondrial respiratory rates are low, averaging 0.006 uaO (uatom oxygen per mg protein per min). The addition of succinate stimulates respiration to 0.016 uaO and addition of ADP as high energy phosphate acceptor further stimulates respiration to 0.07 uaO. The respiratory control ratios (RCR, respiration with ADP/ respiration without) are over 5 in the most satisfactory preparations. KCN (.1 mM), antimycin A (0.5 μg/mg pr) and malonate (3 mM) inhibited succinate respiration. DPNH-supported respiration is low unless cytochrome c is added. TSH does not augment respiration with succinate, alpha-ketoglutarate, TPNH, or DPNH, with or without cytochrome c. Methimazole (10 mM), KCIO4 (1 mM) and KI (10 mM) do not alter respiratory activity. Mitochondrial ATPase activity tends to exceed acceptorless succinate oxidation by a ratio of 3.7:1. DNP, 0.1...
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