Abstract
The iodinated amino acids of the thyroactive iodinated casein, Protamone, were separated. With two-dimensional thin-layer chromatography, at least 21 iodinated components were demonstrated in the initial n-butanol extract of Protamone hydrolysate. Four of these compounds remained when the alkali-washed hydrolysate was also chromatographed two-dimensionally. Two of these four compounds were identified as thyroxine and triiodothyronine. The concentrations of these two compounds in Protamone were determined to be 0.79 and 0.61%, respectively. The combination of these two values is biologically equivalent in the rat to 3.28% thyroxine. It is concluded that the combined thyroxine and triiodothyronine content of thyroactive iodinated casein is sufficient to account for all of its thyroidal activity in mammals.
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