Abstract

Chick embryo thyroid fragments were cultivated in special tissue culture chambers designed primarily for cytological investigations of the living cell. Employing time-lapse techniques three secretory phenomena were observed. These were associated with (1) cytoplasmic droplets which were equivocally PAS positive and (2) cytoplasmic phase contrast green granules, thelocalization sites for I131. Chicken whole egg ultrafiltrates stimulated the epithelial outgrowths to a marked degree but this was not apparent for the fibroblastoid stromal elements.

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