Thymus daenensis extract and essential oils effects on morphine withdrawal signs in mice.

Mohammad Javad Khodayar,Zahra Mansourzadeh,Seyed Amir Hossein Tabatabaei,Amir Siahpoosh,Esmaeil Taherzadeh

doi:10.17795/jjnpp-9959

Abstract

Thymus species are well known medicinal plants which the previous studies suggested the involvement of the opioid system in them. This study aimed to investigate the effects of methanolic extract and essential oil of aerial parts of Thymus daenensis (TD), an endemic aromatic medicinal plant of Iran, on morphine withdrawal syndrome in mice. Experiments were performed in two groups of five, each group treated with extracts or essential oils of TD. Dependency was induced by subcutaneous injection of morphine for three consecutive days. On the fourth day, the last dose of morphine was injected two hours prior to intraperitoneal injection of naloxone while the extract or essential oil of TD was administered 30 minutes before naloxone. A period of 20 minutes after naloxone injection was considered the critical period of the withdrawal syndrome. The number of jumps, standing, leaning, and the weight of stools were recorded as withdrawal signs. The 200 mg/kg and 400 mg/kg doses of extract and all doses of essential oil decreased significantly the number of jumps, standing, leaning and the weight of stool. Administration of 100 mg/kg of extract only decreased the weight of stool and had no effect on the other factors. Extract and essential oil of TD attenuates morphine withdrawal behaviors in mice and may be useful in alleviating the signs and symptoms of opiate withdrawal syndrome in human.

Highlights

Thymus species are well known medicinal plants which the previous studies suggested the involvement of the opioid system in them
Different doses of extract (100, 200 and 400 mg/kg) and essential oil (0.5, 1, and 2.5 mL/kg) of Thymus daenensis (TD) decrease the amount of defecation in morphine-dependent mice precipitated by administration of naloxone (5 mg/kg, IP) 2 hours after the last dose of morphine
The present study examined the effect of TD on naloxone-induced behaviors such as jumping, defecation, standing and leaning immediately after injection over a 20-minute period

Summary

Introduction

Thymus species are well known medicinal plants which the previous studies suggested the involvement of the opioid system in them. Objectives: This study aimed to investigate the effects of methanolic extract and essential oil of aerial parts of Thymus daenensis (TD), an endemic aromatic medicinal plant of Iran, on morphine withdrawal syndrome in mice. Results: The 200 mg/kg and 400 mg/kg doses of extract and all doses of essential oil decreased significantly the number of jumps, standing, leaning and the weight of stool. An important question is whether nonaddictive, non-opioid drugs, can satisfactorily substitute for maintenance therapy in treating opponent processes and craving. In this regard, treatment of addicts with nonopioid drugs, especially natural plant products represent another type of intervention [5]

Objectives

Methods

Results

Conclusion