Abstract
To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.
Highlights
Abiotic stress-related research in plants has considerably increased in recent years as a result of the constant change in the global climate conditions [1,2]
Arabidopsis thaliana [6,7,8,9,10,11,12,13], Camellia Japonica [14,15,16,17], Oryza sativa [18], Nicotiana tabacum [19,20], Phalaenopsis Chiada Pioneer [21], and Physcomitrella patens [22] were the plants that have been employed in various microfluidic platforms for in-depth optical analysis of the dicot seed germination, leaf development, cell phenotypes, protoplasts, pollen tube development and dynamics, shoot and the root growth
Earlier microscopic studies have been done on the morphology [32,33], growth [34] and development [35] of Brachypodium and our study focuses on the real-time growth dynamics and drought conditions in young seedlings
Summary
Abiotic stress-related research in plants has considerably increased in recent years as a result of the constant change in the global climate conditions [1,2]. Arabidopsis thaliana [6,7,8,9,10,11,12,13], Camellia Japonica [14,15,16,17], Oryza sativa [18], Nicotiana tabacum [19,20], Phalaenopsis Chiada Pioneer [21], and Physcomitrella patens [22] were the plants that have been employed in various microfluidic platforms for in-depth optical analysis of the dicot seed germination, leaf development, cell phenotypes, protoplasts, pollen tube development and dynamics, shoot and the root growth As summarized, these studies have primarily been carried out in dicot plants and studies on monocot plants are still to be explored
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