Thrombospondin Type-1 Repeat Domain-Containing Proteins Are Strongly Expressed in the Head Region of Hydra.

Kayoko Hamaguchi-Hamada,Mami Kurumata-Shigeto,Manami Sato,Sumiko Minobe,Shun Hamada,Miyuki Matsufuji,Nozomi Fukuoka,Osamu Koizumi,Robert E Steele

doi:10.1371/journal.pone.0151823

Kayoko Hamaguchi-Hamada, Mami Kurumata-Shigeto + Show 7 more

Open Access

https://doi.org/10.1371/journal.pone.0151823

Copy DOI

Journal: PloS one	Publication Date: Apr 4, 2016
Citations: 4	License type: CC BY 4.0

Affiliation: Fukuoka Women's University

Abstract

The head region of Hydra, the hypostome, is a key body part for developmental control and the nervous system. We herein examined genes specifically expressed in the head region of Hydra oligactis using suppression subtractive hybridization (SSH) cloning. A total of 1414 subtracted clones were sequenced and found to be derived from at least 540 different genes by BLASTN analyses. Approximately 25% of the subtracted clones had sequences encoding thrombospondin type-1 repeat (TSR) domains, and were derived from 17 genes. We identified 11 TSR domain-containing genes among the top 36 genes that were the most frequently detected in our SSH library. Whole-mount in situ hybridization analyses confirmed that at least 13 out of 17 TSR domain-containing genes were expressed in the hypostome of Hydra oligactis. The prominent expression of TSR domain-containing genes suggests that these genes play significant roles in the hypostome of Hydra oligactis.

Highlights

Hydra, a member of the phylum Cnidaria, has a simple diploblastic body plan with an oralaboral axis including a head surrounded by tentacles, a body column, and a foot (Fig 1A)
We examined genes selectively expressed in the head region of Hydra oligactis using suppression subtractive hybridization (SSH)
Of the cDNA clones obtained in the present study, 25.3% were derived from genes encoding TSR domain proteins

Summary

Introduction

A member of the phylum Cnidaria, has a simple diploblastic body plan with an oralaboral axis including a head surrounded by tentacles, a body column, and a foot (Fig 1A). Axial patterning processes are continuously active in adult Hydra [1, 2]. The hypostome of Hydra (Fig 1A), the region around the mouth above the tentacle bases, is a key body part for developmental control [4] and the nervous system [5, 6]. The hypostome has been identified as an organizer that controls axial patterning processes in Hydra. Previous studies reported that an isolated piece of the hypostome induced the formation of a secondary axis when it was transplanted into the body column of another Hydra [7, 8]. The mechanisms by which the head organizer induces the secondary axis currently

Methods

Results

Conclusion