Thrombolytic effect of Siddha herbal formulation Sikkanjar Manapagu using in-Silico model

Panneerselvam N R,Subathra T,Anbarasan B

doi:10.47552/ijam.v12i4.2252

Abstract

Background: Thromboembolic disorders are one of the important causes leading to death. In the Siddha system of medicine, many drugs have been mentioned in the literature and their thrombolytic potential needs to be scientifically evaluated. Aim: The study aims to perform the In Silico computational studies of Phytoconstituents of Siddha formulation Sikkanjar Manapagu (SM) to evaluate its thrombolytic potential. Methods: Autodock program was used for the molecular docking studies of the retrieved phytoconstituents such as Zingiberene, Gingerenone-A, 6 Gingerol of Zingiber officinale, Menthol, Luteolin, Citronellol of Mentha arvensis, Eugenol, Limonene, Myrcene, and Linalool of Citrus aurantium against target protein Human Plasminogen Activation Loop Peptide - PDB 4DCB. Results: A total of ten compounds were screened, of these Zingiberene, Menthol, Citronellol, Eugenol, Limonene, Myrcene, and Linalool showed high binding against active amino acid residue 195. Conclusion: Based on further experiments and clinical trials, the formulation Sikkanjar Manapagu could be proved to be effective in thrombolytic treatment.

Highlights

A thrombus is a solid mass formed in blood vessels from the components of blood and is colloquially called a “blood clot”
Autodock program was used for the molecular docking studies of the retrieved phytoconstituents such as Zingiberene, Gingerenone-A, 6 Gingerol of Zingiber officinale, Menthol, Luteolin, Citronellol of Mentha arvensis, Eugenol, Limonene, Myrcene, and Linalool of Citrus aurantium against target protein Human Plasminogen Activation Loop Peptide - PDB 4DCB
Thrombolytic treatment plays a key role in the management of cardiovascular diseases

Summary

Introduction

A thrombus is a solid mass formed in blood vessels from the components of blood and is colloquially called a “blood clot”. Thrombosis occurs due to the imbalance in normal homeostasis in the circulatory system. It is a fatal disease and it can occur from the heart, arteries, and veins or its microcirculation, named cardiac thrombi, arterial thrombi, venous thrombi, and capillary thrombi based on its origin [1, 2]. The broken piece of thrombus in the circulation is “embolus” which may obstruct the blood vessels and the condition is called as “Embolism”. These obstacles hinder the supply of oxygen to the adjacent tissue,. The drug Sikkanjar Manapagu is a syrup-based herbal preparation, mentioned in the Siddha literature. SM has Zingiber officinale, Mentha arvensis, Citrus aurantium, Cicer arietinum, and Saccharum officinarum [9]

Materials and methods

Results and discussion

Conclusion

Conflict of interest Nil