Abstract
Five crotalid venoms were examined for thrombin-like action on prothrombin, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis in conjunction with assays for thrombin and prothrombin. The thrombin-like venom enzymes of Crotalus adamanteus and Agkistrodon contortrix contortrix cleaved specifically the thrombin-vulnerable activation bonds of human prothrombin, but differed from each other and from thrombin in their requirement for phospholipid and Ca ++. In contrast, the thrombin-like venom enzymes of Agkistrodon rhodostoma, Bothrops moojeni , and Bothrops marajoensis had no proteolytic action on human prothrombin. These results are of pharmacologic interest and indicate also the suitability of snake venom enzymes for comparative structure-function studies on the clotting actions of thrombin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
