Thrombin-induced intracellular calcium rise identifies oligodendrocytes derived from SVZ cultures

Abstract

Event Abstract Back to Event Thrombin-induced intracellular calcium rise identifies oligodendrocytes derived from SVZ cultures S. Grade1*, F Agasse1, L-Bernardino C. Gomes1 and Joao O. Malva1 1 University of Coimbra, Center for Neuroscience and Cell Biology, Portugal Glial cell transplantation arises as a promising complementary strategy to challenge oligodendrocytes loss occurring in the demyelinated brain. Recent advances in stem cell biology, namely concerning subventricular zone (SVZ) stem cells have been giving new hopes offering a source of new oligodendrocytes for cell replenishment in MS brains. However, there are yet no good methods for the functional identification of differentiating oligodendrocytes, and pharmacological analysis in these cells prior transplantation.In the present study, we questioned whether SVZ-derived oligodendrocytes could be functionally discriminated on the basis of variations of intracellular calcium levels ([Ca2+]i) following 50 mM KCl, 100 μM histamine or 0,1 U/ml thrombin stimulations. For this purpose, P1-3 C57/BL6 mice SVZ cultures were treated with triiodo-L-thyronine (T3) to promote oligodendrocytic differentiation before functional analysis of the heterogeneous population. Our group has previously shown that SVZ-derived neurons and immature cells can be discriminated due to their selective [Ca2+]i rise upon KCl and histamine stimulations, respectively. Herein, we demonstrate that O4-oligodendrocytes, as observed previously in astrocytes, do not respond to these stimuli. Nonetheless, O4-oligodendrocytes display an increase in [Ca2+]i following thrombin stimulation whereas all the other cell types in the culture were insensitive to thrombin. Thus, oligodendrocytes can be distinguished according to the uniqueness of their response to thrombin.We further show that thrombin-induced calcium increase in SVZ-derived oligodendrocytes is mediated by protease-activated receptor-1 activation with the downstream involvement of Gq/11 and PLC, resulting in the mobilization of calcium from the cellular internal stores.This method allows the analysis of functional properties of the oligodendrocytic cell population in living SVZ cultures which is of major interest for the development of efficient grafting strategies in the demyelinated brain. Furthermore, this technique opens new perspectives for the search of new pro-oligodendrogenic factors to be used prior grafting. Ackowledgments:PTDC/SAU-NEU/68465/2006, FEDER Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Oral Presentation Topic: Symposium 4 – Neuroregeneration Strategies Citation: Grade S, Agasse F, Gomes LC and Malva JO (2009). Thrombin-induced intracellular calcium rise identifies oligodendrocytes derived from SVZ cultures. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Aug 2009; Published Online: 05 Aug 2009. * Correspondence: S. Grade, University of Coimbra, Center for Neuroscience and Cell Biology, Alicante, Portugal, jomalva@fmed.uc.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers S. Grade F Agasse L-Bernardino C Gomes Joao O Malva Google S. Grade F Agasse L-Bernardino C Gomes Joao O Malva Google Scholar S. Grade F Agasse L-Bernardino C Gomes Joao O Malva PubMed S. Grade F Agasse L-Bernardino C Gomes Joao O Malva Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.