Abstract
Three-toed sloth as putative reservoir of Coxiella burnetii, Cayenne, French Guiana.
Highlights
During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne
DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). qPCR targeting the repeated insertion sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8)
The number of IS1111 intergenic sequences found in the genome of strain C. burnetii MST17 was identical to that for the Nine Mile strain
Summary
During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne. DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). We confirmed all positive results by performing a second qPCR DNA samples with Ct values
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