Abstract

Three-toed sloth as putative reservoir of Coxiella burnetii, Cayenne, French Guiana.

Highlights

  • During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne

  • DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). qPCR targeting the repeated insertion sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8)

  • The number of IS1111 intergenic sequences found in the genome of strain C. burnetii MST17 was identical to that for the Nine Mile strain

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Summary

Introduction

During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne. DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). We confirmed all positive results by performing a second qPCR DNA samples with Ct values

Results
Conclusion
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