Abstract
Three-dimensional positioning and orientation of a biological cell under a microscope is an essential step for cell injection and biopsy. The conventional manual cell manipulation and orientation relies on trial and error, with inherent disadvantages such as low efficiency, poor success rate, and inconsistent output. In this paper, we present a system capable of recognizing and adjusting the orientation of a Zebrafish (Danio rerio) embryo in real time with the use of multiple motion control devices. In addition, a custom-made embryo feeding channel and manipulating device was employed to perform cell manipulations in batches. Experimental results demonstrate the system's capability for fast embryo orientation (13 s/cell) with a 97.5% success rate of rotating a cell to a desired orientation about both y - and z -axis of the cell and a low in-plane rotation angle error of 0.5 ${}^{\circ}$ .
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
