Abstract
The aim of this study was to investigate the effect of three-dimensional (3D) bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells (HUMSCs) on cell viability, proliferation and differentiation in vitro. Functional 3D bio-printed microspheres consisting of HUMSCs were constructed using electrostatic inkjet technique. The parameters used for the synthesis of 3D bio-printed tissue constructs were first optimized. The viability, proliferation and differentiation of 3D cultured HUMSCs were assessed. The results of scanning electron microscopy (SEM) showed that isolated HUMSCs exhibited fibroblast-like spindle adherent growth. The optimized printing parameters were 6 kV voltage, 10 mL/h flow, 15 cm receiving height, and alginate: water ratio of 1:1 mixed at 37 °C. Compared with 2D cultured HUMSCs, the 3D cultured HUMSCs have better viability, proliferation and differentiation ability. The results obtained in this study indicate that 3D bio-printed tissue constructs promote HUMSC viability, proliferation, and neural differentiation in vitro.
Highlights
Three-dimensional (3D) printing technology has attracted huge attention in the field of Biomedical Science, and has so far yielded many positive results [1, 2]
Galactosylceramidase (GALC) antibody was purchased from Proteintech (USA); CellTracker CM-DiI dye was a product of Yesen (Thialand), while recombinant human brain-derived neurotrophic factor (BDNF), basic fibroblast growth factor and epidermal growth factor (EGF) were purchased from Peprotech (USA)
Characterization of Human umbilical-derived mesenchymal stem cells (HUMSCs) The results of scanning electron microscopy (SEM) showed that isolated HUMSCs exhibited fibroblast-like spindle adherent growth (Figure1 A)
Summary
Three-dimensional (3D) printing technology has attracted huge attention in the field of Biomedical Science, and has so far yielded many positive results [1, 2]. Bio-electrospraying (BES) technology is an important technique for direct and efficient delivery of cells in the form of a jet Methods such as ink-jet printing (IJP) and aerodynamically-assisted bio-jets (AABJ/T) are used for direct cell handling, they have been shown to reduce cell viability. Human umbilical-derived mesenchymal stem cells (HUMSCs) constitute an attractive source of stem cells for clinical therapies because they are obtained, multipotent, free of ethical or legal conflicts, and show little immunologic rejection [6, 7]. Their slowness to differentiation and low levels of cell aggregation in lesion sites are two important factors that have limited their clinical application. BES technique was used to engineer a functional 3D scaffold for encapsulation of HUMSCs, and the impact of BES technique on their viability, proliferation and differentiation in vitro was investigated
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