Three types of putative presympathetic neurons in the rostral ventrolateral medulla studied with rat brainstem–spinal cord preparation

Abstract

To study the electrophysiological properties of presympathetic neurons in the rostral ventrolateral medulla (RVLM), intracellular recordings were performed by the whole-cell patch-clamp technique. We utilized the neonatal rat brainstem–spinal cord preparation, in which the sympathetic neuronal network is thought to be preserved, unlike in slice preparation. In response to stimulation in the ipsilateral Th 2 spinal segment including intermediolateral cell column (IML), 33 of 151 non-respiratory RVLM neurons showed antidromic action potentials with a constant latency of 45 ms, and can be considered as presympathetic neurons. We classified and characterized the RVLM presympathetic neurons into three types: ‘regularly firing neurons ( n=7)’, which showed ramp depolarization and frequent action potentials (4.2±0.9 spikes/s) with rare excitatory postsynaptic potentials (EPSPs); ‘irregularly firing neurons ( n=21)’, which exhibited many EPSPs that modulated the firing rate; and ‘silent-type neurons ( n=5)’, which discharged action potentials only during current-induced depolarization. Lucifer-Yellow staining showed that the irregularly firing neurons were significantly larger and had more dendrites than the regularly firing neurons. All regularly firing neurons retained their discharges during low-Ca 2+–high-Mg 2+ superfusion that blocks synaptic input, whereas the discharges in 11 of 16 irregularly firing neurons were abolished, suggesting that the regularly firing neurons discharged independently of synaptic input. Seven of 31 RVLM neurons were hyperpolarized by stimulation of vagal afferent nerves. In summary, three types of RVLM presympathetic neurons were characterized by the patch-clamp technique in the brainstem–spinal cord preparation, in which the connection was preserved from vagal afferent to the Th 2 spinal segment through the RVLM. Since antidromic action potentials were demonstrated by stimulation in the Th 2 spinal segment in 33 neurons of all three types, all types of RVLM neurons constitute a part of the sympathetic neuronal network.