Three ribonucleic acids associated with rice stripe virus.

Abstract

Purified rice stripe virus preparation contains three RNA species whose molecular weights were determined as 1.4×106, 1.0×106 and 0.9×106 in the polyacrylamide gel electrophoretic analysis under the 7M urea-denatured conditions. These RNAs were linear molecules with length distribution from 1.2μm to 0.2μm, as observed by electron microscope. RSV was separated into top, middle, sometimes appearing as a peak with a shoulder, and bottom components by sucrose density gradient centrifugation. The top component may be an artifact resulting from degradation during purification. RNA of mol. wt 1.4×106 was associated with the bottom component and RNAs of mol. wt 1.0×106 and 0.9×106 were associated with the middle component apparently consisting of two components, which were not easily separable by sucrose density gradient centrifugation. On the other hand, the RNA of top component seemed to be degraded into various sizes.