Abstract
The sex-typing technique described allows detection of conventionally used fragments from first intron, shortened by Alonso to the 62/68 bp (for the X and Y, respectively) and fluorescently labelled. Additionally, two mutation points along the fragments, which differs for the X and Y copies, are detected with minisequencing. The challenge compromises also possibility of single injection analysis of fluorescently labelled fragments and mutation points with the use of the same matrix/filter set. In the present study, the new developed technique for gender-typing was tested on fresh and partially degraded forensic samples.
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