Abstract
Microcircular planar chromatography (μ-PLC) is possible without any mobile phase tank. It also offers a way to use a quite strong room temperature air or gas flushing mode to remove carefully and completely cleaning liquids, sample solvent traces, focusing liquids from the stationary phase in order to let PLC run under fully controlled mobile and stationary phase conditions. This mode of circular PLC opened a simple way to introduce a third chromatography phase, which is gaseous and can be loaded with selectivity changing substances like acids, bases, or volatile organic substances of any polarity. All phase flow is constant per time. This offers accuracy and precision of analytical data unknown in thin-layer chromatography up to now. The Three Phase chromatography works already with a rather simple instrumentation — comparable to the instrumental mode of μ-PLC [1].
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