Abstract
Diversity of Creptotrema was investigated using morphological and molecular tools, including data for Creptotrema creptotrema (type-species). Three new species, parasites of Brazilian fishes, are described: Creptotrema conconae n. sp. (type-host, Imparfinis mirini Haseman), Creptotrema schubarti n. sp. (type-host, Characidium schubarti Travassos) and Creptotrema megacetabularis n. sp. (type-host, Auchenipterus osteomystax (Miranda Ribeiro)). The diagnosis of the genus was amended to include new features. The new species differ from each other mainly in terms of body shape, relative sucker size, and testes position. DNA sequences were obtained from Creptotrema spp. from Brazil, including 28S, ITS and COI. Genetic divergences among the new species and C. creptotrema varied from 2.1 to 5.2% (21–49 bp) for 28S, and 6.6 to 16.4% (21–45 bp) for COI. Phylogenetic analysis (28S) placed the newly generated DNA sequences of Creptotrema in a clade (Creptotrema clade sensu stricto) composed of C. creptotrema, the new species described herein, and all species previously described as Auriculostoma, revealing that Auriculostoma is best interpreted as a synonym of Creptotrema based on the principle of priority of zoological nomenclature. Creptotrema funduli, the single sequence of the genus previously available, was not grouped within the Creptotrema clade sensu stricto, suggesting the need for reevaluation of the taxonomic status of this species. Our results showed that Creptotrema represents a monophyletic genus of trematodes widely distributed across the Americas, which currently comprises 19 valid species of parasites of teleosts and anurans.
Highlights
Allocreadiidae Looss, 1902 includes relatively small digeneans that appear to be restricted to freshwater systems distributed across the Americas, Asia, and Europe [11, 24]
Creptotrema Travassos, Artigas & Pereira, 1928 was described to accommodate the type-species Creptotrema creptotrema Travassos, Artigas & Pereira, 1928 as allocreadiids presenting one pair of muscular ventrolateral papillae associated with the oral sucker and a uterus that does not reach the posterior region of the testes
The accurate morphological analyses of type-specimens of Creptotrema deposited in museums and the phylogenetic positioning among isolates provided in this study allowed us to formally describe three new species and to amend the diagnosis of Creptotrema
Summary
Allocreadiidae Looss, 1902 includes relatively small digeneans that appear to be restricted to freshwater systems distributed across the Americas, Asia, and Europe [11, 24]. Creptotrema Travassos, Artigas & Pereira, 1928 was described to accommodate the type-species Creptotrema creptotrema Travassos, Artigas & Pereira, 1928 as allocreadiids presenting one pair of muscular ventrolateral papillae associated with the oral sucker and a uterus that does not reach the posterior region of the testes. Species of this genus parasitize freshwater teleosts belonging to the family Anostomidae in Brazil. There are currently seven valid species in this genus [26] (excluding Creptotrema agonostomi Salgado-Maldonado, Cabañas-Carranza & Caspeta-Mandujano, 1978, recently reassigned as Pseudoparacreptotrema Pérez Ponce de León, Pinacho-Pinacho, Mendoza-Garfias, Choudhury & GarcíaVarela, 2016 [46]), described as parasites of teleosts in the United States, Brazil, Ecuador, Paraguay, and Argentina, and toads (Bufonidae) from Colombia
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
