Abstract
Three new oleanane-type triterpenoidal glycosides, imbalosides A–C (1–3), were isolated from the white flowers of Impatiens balsamina. The structures of these phytochemical constituents (1–3) were elucidated through 1D and 2D Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) data analyses followed by chemical methods. All the characterized compounds (1–3) were evaluated for their antiproliferative activity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and BT549) and their anti-neuroinflammatory activity on the basis of inhibition levels of nitric oxide (NO) in the lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cell lines.
Highlights
Impatiens balsamina L., known as garden balsam or rose balsam, is an annual plant belonging to the family Balsaminaceae and is widely distributed in Korea, Japan, and mainland China
Inspection of the 1 H and 13 C Nuclear Magnetic Resonance (NMR) data of 3 proposed that these data closely resembled those of 2, with a noticeable difference of the presence of one set of resonances attributable to a xylopyranose unit [δH 4.55, 3.84, 3.49, 3.35, 3.26, and 3.17; δC 106.5, 77.9, 76.5, 71.4, and 67.3] with a β-configuration suggested by the large coupling constant (7.4 Hz) between H-1000 and H-2000 (Table 1)
The location of this additional xylopyranose unit was determined by the Heteronuclear Multiple Bond Correlation (HMBC) cross-peak of H-1000 to
Summary
Impatiens balsamina L., known as garden balsam or rose balsam, is an annual plant belonging to the family Balsaminaceae and is widely distributed in Korea, Japan, and mainland China. Diverse parts of I. balsamina, including flowers, stems, and leaves, have long been used as traditional medicines to treat various diseases. As part of the continuing studies to identify the bioactive constituents from Korean medicinal plants [6–11], we previously conducted a phytochemical investigation on the MeOH extract of the white flowers of I. balsamina, which led to the isolation and characterization of phenolic compounds including mono- and biflavonoids with cytotoxic, anti-inflammatory, and neuroprotective activities [12,13]. The isolates [1,2,3] were tested for their cytotoxicity against four human tumor cell lines and anti-neuroinflammatory activity using lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cell lines. Plants 2020, 9, x FOR PEER REVIEW cytotoxicity against four human tumor cell lines and anti-neuroinflammatory activity using lipopolysaccharide murinecell microglia. Plants 2020, 9, 1083 lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cell lines
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