Abstract
The medicinal plant Tirpitzia sinensis has been used by the Zhuang ethnic people in mountainous areas of Southwest China to stop bleeding, invigorate blood circulation, and treat inflammation and wounds. In order to further explore its traditional medicinal uses, the phytochemical constituents of this species were examined. Three new compounds, the lignan tirpitzin (1), the flavonoid tirpitzoside (2), and the furan-glycoside tirpitziol (3), along with five known compounds were isolated from the aerial part of T. sinensis for the first time. The structures of these compounds were elucidated by 1D and 2D NMR, LC/MS, IR spectrometric methods and compared with published data. The results of an in silico pharmacophore-based analysis showed potential targets of the new compounds, including ERBB2, IRAK4, LCK, JAK2, MAPK14, and MMP-12. These targets suggested that 1–3 may be involved with wound-healing and/or inflammation, leading to an in vitro assay of nitric oxide (NO) inhibition assays with lipopolysaccharide-induced BV-2 cells. All three new compounds displayed moderate NO inhibitory activity with the IC50 values of 14.97 ± 0.87, 26.63 ± 1.32, and 17.09 ± 2.3 μM, respectively.
Highlights
Tirpitzia sinensis (Hemsl.) Hallier f., known in China as “qing li chai”, is a shrub or small tree in the Linaceae family
*Correspondence: edward.kennelly@lehman.cuny.edu; long@mail.kib.ac.cn; long.chunlin@muc.edu.cn 1 College of Life and Environmental Sciences, Minzu University of China, 27 Zhongguancun South Ave., Haidian, Beijing 100081, People’s Republic of China 3 Department of Biological Sciences, Lehman College, City University of New York, 250 Bedford Park Boulevard West, Bronx, New York 10468, USA Full list of author information is available at the end of the article of Jingxi County in Guangxi during 2012 and 2013, we found that the Zhuang people used T. sinensis as a medicinal plant to stop bleeding, invigorate blood circulation, and treat inflammation and traumatic injury
The presence of a doublet at δH 5.53 (1H, d, J = 6.2 Hz, H-7) and three characteristic carbon signals at δC 89.3 (C-7), 55.5 (C-8) and 65.0 (C-9) indicated that compound 1 belongs to a group of dihydrobenzofuran-type lignans, which was confirmed by 1H–1H correlation spectroscopy (COSY) correlations of H-7/H-8/H-9 (Fig. 3)
Summary
Tirpitzia sinensis (Hemsl.) Hallier f., known in China as “qing li chai”, is a shrub or small tree in the Linaceae family. It is distributed mainly on the geologically distinct highly exposed karst limestone hills and low mountains in Guangxi, Guizhou, and Yunnan provinces of Southwest China and northern Vietnam. Screening the bioactivity of T. sinensis constituents is a first step to evaluate scientifically the rationale of its traditional medicinal uses. Biological screening can be complex since the assay system to be used is not always obvious. The assays chosen can be time- and cost-intensive and the success rate may not be high [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
