Three new compounds isolated from the bulbs of Fritillaria pallidiflora Schrenk and their anti-inflammatory activity

Abstract

• Three new compounds were isolated from the bulbs of Fritillaria pallidiflora Schrenk. • Their structure were characterized by extensive 2D-NMR studies. • Their anti-inflammatory activities were also evaluated. Three new compounds, 17 β -cevanin-6-oxo-5 α ,20 β -diol yibeinine ( 1 ), 2-(tetrahydro-5-(2-hydroxyphenyl)-2H-pyran-3-yl) phenol ( 2 ), 1,3- O -diferuloyl-2-methoxypropane diol ( 3 ), as well as four known compounds ( 4 – 7 ), have been isolated from the ethanol extract of dried bulbs of Fritillaria pallidiflora Schrenk. All structures were determined based on their spectroscopic data (1D and 2D NMR (including 1 H NMR, 13 C NMR, 1 H- 1 H COSY, HMBC, HSQC, HSQC-TOCSY, and NOESY experiments), and MS). Biological evaluation showed that compounds 1 – 4 inhibited the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells with IC 50 values of 18.0, 38.7, 29.5, and 47.1 μM, respectively. These results indicated that compound 1 has potential anti-inflammatory activity.