Three endogenous cellulases from termite, Reticulitermes speratus KMT001.

Abstract

Among termites, lower termites need symbiotic microorganisms in the digestive tract for digestion and cellulose metabolism. In this symbiotic relationship, the decomposition of cellulose is initiated by endoglucanase in termite salivary glands and completed by β-glycosidase of symbiotic microorganisms in the hindgut. The expression of β-glycosidase in lower termites has been reported in recent studies. The expression of two endoglucanases and one β-glycosidase generelated to cellulose degradation was identified in Reticulitermes speratus, a lower termite, through transcriptomic analysis. The proposed enzyme activities of three identified cellulose degradation genes were confirmed by heterologous expression in Escherichia coli. In addition to the endoglucanase expressed in the salivary gland, additional endoglucanase and β-glycosidase genes suggest that R. speratus performs the overall cellulose digestion using its own enzymes at all stages.