Abstract
Ala-Pro (AP), Ile-Pro-Ala (IPA) and Ile-Pro-Ala-Val-Phe (IPAVF) as DPP-IV inhibitory peptides were purified from Antarctic krill protein hydrolysate (AKH). Diabetic zebrafish model was rapidly induced with combination of high glucose immersing and cholesterol diet for evaluation the three DPP-IV inhibitory peptides activity. Physiochemical indexes including DPP-IV activity, glucose, triglyceride and cholesterol; the relative gene (insa, glucagon and pck1) expressions levels were detected. The results showed that after 15 days of peptides treatment, the physiochemical index levels of peptide groups were significantly higher than that of negative control and showed dose-dependent effect. The insa gene expression level would be increased with the enhancement of peptide concentration, whereas gene expression levels of glucagon and pck1 would be decreased. These results indicated that three peptides all had hypoglycemic effects in different degree. It suggested the potential of AKH containing DPP-IV inhibitory peptides could be as functional food supplement to treat diabetes.
Highlights
Type 2 diabetes is a chronic metabolic disorder characterized by high blood glucose due to insufficient insulin secretion and insulin resistance
The aim of the present study is to set up a diabetic zebrafish model and investigate putative glycemic effects of three novel DPP-IV-inhibiting peptides isolated from Antarctic krill protein hydrolysates
Excess glucose in aquatic environment in zebrafish induces symptoms related to the pathophysiology of diabetes, supporting the utility of glucose/cholesterol exposure to induce hyperglycemic diabetic-like states (Gleeson et al, 2007)
Summary
Type 2 diabetes is a chronic metabolic disorder characterized by high blood glucose due to insufficient insulin secretion and insulin resistance. This disorder is widespread, severely debilitating, and affects a large portion of the global population (Thomas et al, 2019). Recent focus on nutrition has shifted towards searching for novel therapies and diets to treat and prevent metabolic disorders, including diabetes. Antarctic krill (Euphausia superba) has 3-10 billion fishing tons annual globally It contains 11.9-15.4% proteins (Ji et al, 2017) that include all essential amino acids, and is enriched in proline, methionine, leucine and lysine (Wang et al, 2015; Sun & Mao, 2016). It’s a good resource for extracting bio-active peptides, such as DPP-IV inhibitor for diabetes, for nutritional or medical purposes
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