Three-dimensional structure of the gustatory cell in the mouse fungiform taste buds: a computer-assisted reconstruction from serial ultrathin sections.

Abstract

Taste buds in fungiform papillae of the mouse were examined with transmission electron microscopy and computer-assisted, three-dimensional reconstruction from serial ultrathin sections. In accord with observation by Murray (1971), four distinct cell types, type I, II, III and basal cells, were identified. Of these, only the type III cell made synaptic contacts with nerve terminals and contained both small, clear vesicles and dense-cored granules. The former vesicles were synaptic-type and accumulated in the cytoplasm just below the synaptic in membrane thickenings. This finding clearly indicates a sensory function for the type III cell. One to three type III cells were identified within a taste bud. The type III cell had at most eight synapses with nerve terminals. One nerve fiber making two synapses with the type III cell was occasionally observed in its terminal region.