Abstract
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.
Highlights
Positron Emission Particle Tracking (PEPT) was first proposed in a US Patent by Shaw in 1978 [1]
We report our imaging protocol and PEPT method, along with yeast cell tracking results
A method for multiple particle tracking via PEPT is presented based on optical feature point identification methods
Summary
Positron Emission Particle Tracking (PEPT) was first proposed in a US Patent by Shaw in 1978 [1]. The majority of PEPT evaluations have used the Birmingham method [2] and tracked a single particle. Bickell et al [7] introduced a separate method for PEPT processing that allowed for tracking multiple particles of known initial positions. Wiggins et al [8] advanced this method to track an arbitrary number of particles of unknown initial positions. This has since been used to examine flows where particles routinely pass into and out of the field of view of the detection system [9, 10]. Another adaptation of the method of Bickell et al is introduced in [11] and is described and used
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