Abstract
Changes in microcirculation are believed to perform an important role after cardiac arrest. In particular, rheological changes in red blood cells (RBCs) have been observed during and after ischemic-reperfusion injury. Employing three-dimensional laser interferometric microscopy, we investigated three-dimensional shapes and deformability of RBCs during and after asphyxial cardiac arrest in rats at the individual cell level. Rat cardiac arrest was induced by asphyxia. Five rats were maintained for 7 min of no-flow time, and then, cardiopulmonary resuscitation (CPR) was started. Blood samples were obtained before cardiac arrest, during CPR, and 60 min after return of spontaneous circulation (ROSC). Quantitative phase imaging (QPI) techniques based on laser interferometry were used to measure the three-dimensional refractive index (RI) tomograms of the RBC, from which structural and biochemical properties were retrieved. Dynamic membrane fluctuations in the cell membrane were also quantitatively and sensitively measured in order to investigate cell deformability. Mean corpuscular hemoglobin, mean cell volume, mean corpuscular hemoglobin concentration, and red blood cell distribution width remained unchanged during CPR and after ROSC compared with those before cardiac arrest. QPI results revealed that RBC membrane fluctuations, sphericity, and surface area did not change significantly during CPR or after ROSC compared with initial values. In conclusion, no three-dimensional shapes and cell deformability changes in RBCs were detected.
Highlights
Sudden cardiac arrest is one of the main causes of death worldwide
All rats were maintained for 7 min of no-flow time after mean arterial pressure was
Blood samples were obtained before cardiac arrest, during CPR, and 60 min after return of spontaneous circulation (ROSC) to check arterial blood gases and CBC
Summary
Sudden cardiac arrest is one of the main causes of death worldwide. For decades, various research efforts have improved the survival of OHCA victims. Several recent studies have reported an essential role of RBCs in the modulation of the microcirculatory dysfunctions [6,7,8,9,10,11,12,13]. Ese changes contribute the microcirculatory dysfunction and the progress of the tissue injury [11, 17]. Cardiac arrest survivors suffer from global IR injury, and microcirculatory changes have important roles in the pathologic process of cardiac arrest survivors. Rheological properties of RBCs were addressed by analyzing dynamic membrane fluctuations in a cell membrane With this experiment, we wanted to evaluate the membrane properties, structures, and functional status of RBCs after cardiac arrest
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
