Three-dimensional quantitative phase imaging of blood coagulation structures by optical projection tomography in flow cytometry using digital holographic microscopy.

Hideki Funamizu,Yoshihisa Aizu

doi:10.1117/1.jbo.24.3.031012

Hideki Funamizu, Yoshihisa Aizu

Open Access

https://doi.org/10.1117/1.jbo.24.3.031012

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Abstract

Blood coagulation is an important role in the hemostasis process. In the observation using microscopies, an aggregation structure of red blood cells indicates the degree of blood coagulation. Recently, it has been proposed that digital holographic microscopy (DHM) is a powerful tool for biomedical cell imaging on the basis of quantitative phase information. DHM has the advantage in that the real-time and three-dimensional (3-D) quantitative phase imaging can be realized in the wide field of view, which means that the 3-D morphological parameters of biological cells without a staining process are obtained in real time. We report the complete 3-D quantitative phase imaging of blood coagulation structures by optical projection tomography in a flow cytometry using DHM.

Highlights

It is known that blood coagulation is an important role in hemostasis related to the cessation of blood loss from a damaged blood vessel
digital holographic microscopy (DHM) has the advantage in that a thickness of biological cells, which is regarded as the depth information in an observation of cell morphology using quantitative phase microscopies in many cases, is obtained from a single hologram and the real time and 3-D quantitative phase measurement can be realized in the wide field of view
In the process of optical projection tomography, the number of holograms, which corresponds to the number of projected phase maps, is 92, 46, and 51 for a single red blood cells (RBCs) and the blood coagulation structures composed of three and nine RBCs, respectively

Summary

Introduction

It is known that blood coagulation is an important role in hemostasis related to the cessation of blood loss from a damaged blood vessel. It has been proposed that digital holographic microscopy (DHM) is a powerful tool for biomedical cell imaging, and it is actively applied to the blood testing on the basis of quantitative phase information.[11,12,13,14,15] DHM has the advantage in that a thickness of biological cells, which is regarded as the depth information in an observation of cell morphology using quantitative phase microscopies in many cases, is obtained from a single hologram and the real time and 3-D quantitative phase measurement can be realized in the wide field of view It means that the 3-D morphological parameters and activities of biological cells without a staining process can be obtained using DHM in real time. This paper is the first report of the 3-D tomographic phase imaging of blood coagulation structures using DHM

Optical Setup

Results and Discussion

Conclusion