Abstract
Recent advances in NMR microscopy are discussed with applications to the study of single cell development. Fundamental resolution limit due to molecular diffusion as well as experimental resolution limits arising from system bandwidth and poor signal-to-noise ratio due to small image voxel size arc studied. Finite line broadening due to the signal attenuation by diffusion are also investigated. Several microscopic imaging pulse sequences to maximize signal-to-noise ratio as well as tissue dependent contrast are presented and applied to elucidate cellular imaging such as the cell lineage patterns in Xenopus luevis embryos. Experimental results obtained with 7.0 Tesla NMR microscopy system are presented.
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