Abstract
Herein, we reported the design of a highly sensitive electrochemical immunosensor and its application for early diagnosis of acute myocardial infarction (AMI) with the biomarker of cardiactroponin I (cTnI), which was based on a novel three-dimensional nano-network composed of Pt nanoparticles functionalized Mn- doped CeO2 nanoparticles (Pt@Mn-CeO2 NPs) and hemin/G-quadruplex as electrocatalysts and biolabels for signal amplification. The synthesis of three-dimensional nano-network involves self-assembly of specially designed DNA sequences on the surface of Pt@Mn-CeO2 NPs. And on the basis of it, the immunosensor showed a wide linear range from 0.5pgmL−1 to 100ngmL−1 and a low detection limit of 0.15pgmL−1 through sandwich immunoreactions. We believe the high sensitivity could attribute to the factors of (1) the G-rich sequences on three-dimensional nano-network of Pt@Mn-CeO2 NPs could embed masses of hemin as redox mediator and electrocatalysts; (2) the formation of the new synergistic pseudobienzyme-catalyzed cascade system based on hemin/G-quadruplex and Pt@Mn-CeO2 NPs could amplify the electrochemical signal in the presence of the l-cysteine, which was the electrocatalytic substance. Furthermore, the combination of nanocatalyst and DNAzyme also provided a promising signal amplification strategy for designing smart biosensors for other analytes detection (e.g., DNA, RNA, metal ion, peptide).
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