Abstract
A three-dimensional (3D) laser-scanning confocal microscope was developed for multicolor 3D co-localization of single fluorescent molecules immobilized at a temperature of 1.5K. The image quality was tested by taking 3D emission image of single quantum dots with three different excitation wavelengths of 635, 532 and 405nm. The 3D image size was all within 1.2 times of the diffraction-limited size. From the centroid position of the 3D image taken with 635- and 532-nm excitations, 3D co-localization precision of single dot was determined to be 3nm in the lateral (x, y) and 18nm in the axial (z) directions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
