Abstract
Three-dimensional (3-D) image performance of confocal single-photon and two-photon fluorescence microscopy under ultrashort pulse illumination is analysed in terms of the 3-D optical transfer function. It is found that the material dispersion does not affect the imaging performance if a well-corrected commercial objective lens is used. The finite spectral bandwidth of an ultrashort pulse of a temporal duration shorter than 10 fs may result in significant improvement in resolution. The improvement becomes less pronounced in two-photon fluorescence imaging.
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