Abstract
Using serial focussing transmitted light through a differential interference contrast (DIC) and three-dimensional image analysis, the orientation and the structural changes of normal and abnormal prisms were observed in tangential ground sections of human enamel. The outer layer of permanent enamel consisted of a mild undulation of enamel prisms. The neonatal line of deciduous enamel showed a change in the prism arrangement. The alternation of a constriction and a varicosity with a periodicity of about 3-5 μm was identified as the cross-striations of prisms. Double bordered and spiral prisms in the inner layer of permanent enamel temporarily appeared on the way to the formation of normal arcade prisms. Stunted prisms in the outer layer, however, maintained the shape during a relatively long distance.
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