Abstract
There is a limited number of established ovarian cancer cell lines matching the low-grade serous histotype available for research purposes. Three-dimensional (3D) culture systems provide in vitro models with better tissue-like characteristics than two-dimensional (2D) systems. The goal in the study was to characterize the growth of a given low-grade serous ovarian carcinoma cell line in a 3D culture system conducted in a magnetic field. Moreover, the culture system was evaluated in respect to the assembly of malignant cell aggregates containing lymphocytes. CAISMOV24 cell line alone or mixed with human peripheral blood mononuclear cells (PBMC) were cultured using a commercially available 3D culture system designed for 24 well plates. Resulting cell aggregates revealed the intrinsic capacity of CAISMOV24 cells to assemble structures morphologically defined as papillary, and reflected molecular characteristics usually found in ovarian carcinomas. The contents of lymphocytes into co-cultured cell aggregates were significantly higher (p < 0.05) when NanoShuttle-conjugated PBMC were employed compared with non-conjugated PBMC. Moreover, lymphocyte subsets NK, T-CD4, T-CD8 and T-regulatory were successfully retrieved from co-cultured cell aggregates at 72h. Thus, the culture system allowed CAISMOV24 cell line to develop papillary-like cell aggregates containing lymphocytes.
Highlights
Epithelial ovarian cancer (EOC) is among the most lethal gynecological malignancies, ranking third as a cause of women’s worldwide deaths
The first 3 h under magnetic field promoted by the bioprint drive brought CAISMOV24 cells together in a round-shaped structure
Histological cuts the presence of focal acinar arrangement with secreted material, pointing out that 3D culture system revealed the presence of focal acinar arrangement with secreted material, pointing out that 3D culture enabled CAISMO24 cells to evolve glandular-like structures (Figure 1e)
Summary
Epithelial ovarian cancer (EOC) is among the most lethal gynecological malignancies, ranking third as a cause of women’s worldwide deaths. Surgery can cure most treated women when the malignancy is still restricted to the ovaries, but 79% of women with EOC are diagnosed at advanced stages, resulting in a poor five years survival rate of 20–25% [1,2,3]. Ninety percent of the ovarian malignancies are of epithelial origin and comprise four main histotypes. EOCs are further categorized based on their growth and molecular characteristics as type I or II. The serous histotype comprises 70% of all EOC, among which the type II or high-grade, accounts for two-thirds of all ovarian cancer deaths [7,8,9]
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